THE PURIFICATION OF COENZYME A BY ION EXCHANGE CHROMATOGRAPHY
نویسندگان
چکیده
منابع مشابه
The purification of coenzyme A by ion exchange chromatography.
With the increasing demand for coenzyme A (CoA) to be used as a substrate in enzyme studies, a need has developed for additional methods of isolating this substance from natural sources. Two methods have been published whereby CoA of reasonably high purity can be prepared (1, 2). Both of these procedures are lengthy and the over-all yields are relatively poor (10 to 30 per cent). One of these m...
متن کاملPurification of bradykinin by ion-exchange chromatography.
Shirai, Y. & Ohkubo, T. (1954a). J. Biochem., Tokyo, 41, 337. Shirai, Y. & Ohkubo, T. (1954b). J. Biochem., Tokyo, 41, 341. Storey, I. D. E. (1950). Biochem. J. 47, 212. Storey, I. D. E. & Dutton, G. J. (1955). Biochem. J. 59,279. Storey, I. D. E. & Dutton, G. J. (1956). Proc. 3rd Int. Congr. Biochem., Brussel8, 1955, p. 162. Strominger, J. L., Kalckar, H. M., Axelrod, J. & Maxwell, E. S. (1954...
متن کاملpurification of human serum albumin by ion exchange chromatography
introduction : albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. we compared two different methods for albumin purification: carboxymethyl cellulose (cm cellulose) resin exchange and diethylaminoethyl cellulose (deae cellulose) resin exchange in order to determine which resin could be more beneficial. materials and methods : two ion exchang...
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Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification of EVs. EVs in bulk were isolated from pooled normal human amniotic fluid (AF) by differential centrifugation followed by IEC or sucrose densi...
متن کاملIsolation and purification of albumin from human plasma by direct and combined approach of ion-exchange chromatography and comparison of the final products quality obtained by both methods
Background: Due to multiple roles of albumin in the body, injection of its medicinal product as one of the therapeutic or management strategies under conditions such as severe bleeding, burns, liver failure, and neonatal hemolytic diseases is on the physicians' agenda. Considering that albumin is the most abundant plasma protein, designing an appropriate method to purify it is highly important....
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52612-6